Jason MarksIn January 2019, MaxCyte was excited to announce two new processing assemblies designed to further enhance experimentation flexibility, workflow and transfection efficiency, and cell recovery effectiveness.

Jason Marks — Marketing Manager, Strategic Products, discusses the advantages of these two new additions to the MaxCyte product portfolio.

What are the new products MaxCyte introduced?

The OC-25×3 and OC-100×2 are the first multi-well processing assemblies available for use with MaxCyte’s Expert platform. The OC-25×3 has three wells with a 25 uL volume per well. Each well can hold 125,000 to 5 million cells, and each cuvette can hold 375,000 to15 million cells. In comparison, the OC-100 x2 has a two-well configuration, with a 100 uL volume per well. Each well can hold 500,000 to 20 million cells, totaling one to forty million cells per cuvette.

What are the advantages of the new processing assemblies?

Unlike our previous single-well cuvettes, the multi-well configuration allows users to run multiple samples concurrently. This capability saves a lot of time, increases sample throughput, and improves workflow efficiency. Additionally, the multiple wells allow more experiments to be run in a shorter timeframe, leading to a much lower cost per sample transfected. One can simply do much more with the same workflow process.

The OC-25×3 has some additional benefits. Could you explain them?

Cellular therapeutic development is advancing at a rapid pace, often leading developers in new and unexpected directions. Therefore, flexibility is highly valued by these scientists. The OC-25×3 allows scientists to use much smaller volumes of cells than what was feasible with the configurations of our previous process assemblies. Obviously, the flexibility to use volumes as small as 15 uL means that far fewer cells are used and less loading agent is required.

Scientists can now use MaxCyte’s Expert platform in ways that were not possible prior to this advancement. For instance, the new flexibility adds tremendous value when working with difficult-to-collect cells or running experiments on a limited amount of cellular material. Our previous processing assemblies had a capacity of 100 uL, with the option to go as low as 50 uL. However, it is easy to see the added versatility now available to our community of users—it’s exciting!

What are the advantages of the new V-shaped wells?

Previously, the wells within our processing assemblies were square. We listened closely to our customers’ feedback and learned that it was often difficult to remove the cells from the square wells with pipettes. Because cells are frequently quite sticky, the material was prone to “sticking” in the corners.

Particularly when working with small volumes, a scientist needs to recover as many cells as possible. The new V-shaped wells allow cells to be funneled into a smaller space for easier extraction using a pipette, an improvement upon chasing them around four corners of square wells.

Additionally, the V shape contributes to transfection efficiency, particularly when electroporating smaller volumes using the OC-25×3 assembly. To successfully load a cell, the cell must touch the sides of the electrodes. The conical shape funnels the cells into a smaller volume, increasing the likelihood that the cells come into contact with both electrodes and resulting in increased electroporation performance.

Are there more new products in the pipeline for MaxCyte?

I can’t talk about any other new products just yet, but I can tell you that there are some remarkable advancements in the works. Stay tuned!