Decoding Transcriptional Regulation by Genome-Wide Reporter Assays and Large-Scale Transfection Despite our knowledge of the complete genome sequences of several dozen species and high-quality annotation of the protein coding genes, the identification of active regulatory elements remains challenging, especially for distal enhancers. Traditional methods to measure enhancer activity directly are limited by throughput, so conventional approaches depend largely on indirect methods that profile features correlated with regulatory elements including the chromatin landscape and transcription factor binding sites. In this GEN webinar, we will learn about two novel reporter assays, STARR-seq and STAP-seq, ectopic, plasmid-based, massively parallel reporter assays that can directly and quantitatively measure the activity of millions of candidate sequences for enhancers and core promoters. Moreover, we will learn how these reporter assays were enabled by MaxCyte’s scalable flow electroporation technology to attain high transfection efficiencies of plasmid reporter libraries—key to the reporter assays’ success.
Decoding Transcriptional Regulation by Genome-Wide Reporter Assays and Large-Scale Transfectionali2020-09-16T17:16:56+00:00